s1000 touch thermal cycler (Bio-Rad)

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Bio-Rad s1000 touch thermal cycler
S1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s1000+thermal+cyclers/S1000+Firmware+Update+for+Serial+Numbers+SC010000+or+Higher/pmc13019501-85-0-5
Average 99 stars, based on 28 article reviews

s1000 touch thermal cycler - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Characterisation of Four New Genes in the Ovine KAP19 Family
Article Snippet: The thermal cycling was carried out in S1000 thermal cyclers (Bio-Rad, Hercules, CA, USA).

Article Title: Characterisation of the Ovine KRTAP36-1 Gene in Chinese Tan Lambs and Its Impact on Selected Wool Traits
Article Snippet: Thermal cycling was conducted in S1000 thermal cyclers (Bio-Rad; Hercules, CA, USA).

Article Title: Effects of KRTAP20-1 Gene Variation on Wool Traits in Chinese Tan Sheep
Article Snippet: The amplifications were undertaken in S1000 thermal cyclers (Bio-Rad, Hercules, CA, USA) with a preliminary denaturation of 2 min at 94 °C, followed by 35 cycles of 94 °C for 30 s, 60 °C for 30 s, 72 °C for 30 s, and an ultimate extension/polishing step of 5 min at 72 °C.

Polymerase Chain Reaction:

Article Title: Ovine KRTAP36-2 : A New Keratin-Associated Protein Gene Related to Variation in Wool Yield.
Article Snippet: .. The PCR amplifications were undertaken in S1000 thermal cyclers (Bio-Rad, Hercules, CA, USA). ..

Reverse Transcription:

Article Title: Active polypeptide MDANP protect against necrotizing enterocolitis (NEC) by regulating the PERK-eIF2ɑ-QRICH1 axis.
Article Snippet: Then the cells were infected with shRNA lentivirus particles (GeneChem, Shanghai, China) and mixed gently and incubated for 6 h, the cells were incubated with RPMI for 48 h. Subsequently, infected cells were cultured in RPMI medium containing 0.5 μg/mL purinomycin to select stable infected cells expressing QRICH1 shRNA. .. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) Complementary DNA (cDNA) was prepared using qScript cDNA SuperMix (Qiagen, Maryland, USA) and S1000 Thermal Cycles (Bio-Rad Laboratories, California, USA). .. Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Inc., CA, USA).

Real-time Polymerase Chain Reaction:

Amplification:

Article Title: A Preliminary Investigation of Myostatin Gene ( MSTN ) Variation in Red Deer ( Cervus elaphus ) and Its Implications for Venison Production in New Zealand.
Article Snippet: PCR amplification was performed in a 15-μL reaction containing approximately 50 ng of genomic DNA, 0.25 μM of each primer, 150 μM of each dNTP (Bioline, London, UK), 2.5 mM of Mg2+, 0.5 U of Taq DNA polymerase (Qiagen, Hilden, Germany), and 1× reaction buffer supplied with the enzyme. .. The amplification was undertaken in Bio-Rad S1000 thermal cyclers (Bio-Rad, Hercules, CA, USA), and the thermal cycling conditions included an initial denaturation for 2 min at 94 ◦C, followed by 35 cycles of 30 s at 94 ◦C, 30 s at 60 ◦C, and 30 s at 72 ◦C, with a final extension of 5 min at 72 ◦C. ..

Article Title: Variation in ovine KRTAP8-1 affects mean staple length and opacity of wool fiber.
Article Snippet: In this study, keratin-associated proteins gene (KRTAP8-1) from five different sheep breeds and breed-crosses (n1⁄4 310) was genotyped using a Polymerase Chain Reaction-Single Strand confirmation Polymorphism (PCR-SSCP).. Six unique genotypes were observed: AA, AC, AD, AE, DD and EE, with AA being the most common in the different breeds and crosses.. Twelve wool characteristics: yield, mean staple length (MSL), bulk, mean fiber diameter (MFD), fiber diameter standard deviation (FDSD), coefficient of variation of fiber diameter (CVFD), medullation, standard deviation of medullation (MeSD), coefficient of variation of medullation (CVMed), opacity, standard deviation of opacity (OpSD), and coefficient of variation of opacity (CVOp) were measured on wool derived from the sheep.

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